Im am writing the scientific report where I have a calibration curve. I want to use that and find the concentration of an unknown sample. This is easily done by hand, but how do I show it on Excel and on the chart itself?
I need to find x-values according to a four parameter logistic equation ( y = (a-d)/[1+(x/c)^b]+d
How can I do this? I am not very familiar with solver. I have used XLSTAT to find my four parameter curve and my a, b, c, and d values.
To be a little more descriptive (as I am really hoping to get some help here):
I am working with ELISA data. I have a calibration curve, therefore I have concentrations(x, actually in LOG) and their response (y). I will generally get a response (y) from my assay and want to find my estimated concentration according to the calibration curve. (Like using the forecast function, but it is not linear).
I have a theoretical values of three compounds and I got experimental values.
I am trying to graph them and it does not work Here are the values of temp: EXPERIMENTAL: x-axis 80 C
I cannot graph these values. Does anybody have
I am trying to calculate the total time it took to run a batch. I can import the Time and Date of Injection, but the issue is I use a stored calibration curve to calculate concentration and the time and dates of injection of calibration curve are from 1-3 days older. I need for the formula to find the time of the last sample that was injected and subtract that from the time of injection from the first sample named "Blank" to get the total time of the run. I have attached a sample query with notes.
I have discovered how to create a normal distribution (Bell) curve on my excel spreadsheet. The instructions on the net all seem the same, and the curve always covers the same width 1 to 8 or 9. I am trying to produce a curve to lok at a number of ratings the lowest is 0 and the highest 5 How do I amend the width of the bell. I believe it is something to do with the standard deviation number which is generated and used. But now I'm lost
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